Dr. Steeve Boulant

Department of Infectious Diseases, Virology
University Hospital Heidelberg
69120 Heidelberg, Germany

Phone: +49 6221-42 1560
Email:   s.boulant@dkfz-heidelberg.de

FIELDS OF INTEREST

Virus entry, virus-host interactions, anti-viral innate immune eresponse in intestinal polarized epithelium cells, viral commensal intestinal flora, molecular mechanisms triggering viral particle entry, characterization of the dynamic uptake and intracellular trafficking of viral pathogen using live-cell confocal microscopy, quantification of virus-receptor interaction using TORF microscope and characterization of the impact of receptor clustering on virus entry, intracellular locatioan and functional aspects of innate immunity sensor proteins (TLR and RLR) in polarized cells.

CURRENTLY FUNDED PROJECTS

Chica and Heinz Schaller Fundation grant (2012-2017). Currently supervising 1 doctoral thesis, 2 post-doctoral fellows and 1 technician.

AWARDS & HONORS

2011 Post-doctoral fellowship (2011-2012), GlaxoSmithKline, Boston MA, USA
2010 Pilot and Feasiblity award program HDDC, Boston MA, USA
2009 Pilot and Feasiblity award program HDDC, Boston MA, USA
2007 Poster award, FASEB Liqid Droplets conference, Vermont USA
2006 Post-doctoral fellowship Marie-Curie IEF (2006-2008), Glasgow, UK
2006 Best Oral communication, UK Meeting on the Biology and Pathology of HCV
2005 Post-doctoral fellowship (2005-2006), MRC, Glasgow, UK
2005 Travel award, 11th Interantional Meeting on HCV and Related Viruses
2004 Bridging grant, FRM (2004-2005), Lyon, France
2001 EURODOC fellowsgip, Lyon-Glasgow exchange grant

2012-present Group Leader CHS Foundation, University Hospital Heidelberg
2008-2012 Post doctoral associate, Harvard Medical School Boston MA, USA
2006-2008 Marie curie Post doctoral fellow, MRC Virology Unit Glasgow, UK
2005-2006 Post doctoral associate, MRC Virology Unit Glasgow, UK
2004-2005 Bridging grant fellow, IBCP-CNRS Lyon, France
2001-2004 PhD in Molecular Biology and Biochemistry, IBCP-CNRS, France
2000-2001 DEA in Molecular Biology and Biochemistry (Master), Lyon, France
1998-2000 Bachelor degree in Molecular Biology and Biochemistry, Lyon, France
1996-1998 Classe préparatoire Mathematic superior, Lycée Ozenne, Toulouse, France

2013-2016 Chair of the study section “Cell Biology of Viral Infections” of the Society of Virology (GfV)
2010 Co-organisor of live-cell imaging workshop, Harvard Medical School, USA

Pervolaraki K, Stanifer ML, Münchau S, Renn LA, Albrecht D, Kurzhals S, Senís E, Grimm D, Schröder-Braunstein J, Rabin RL, Boulant S (2017). Type I and type III interferons display different dependency on mitogen-activated protein kinases to mount an antiviral state in the human gut. Front Immunol. 21;8:459

Stanifer ML, Rippert A, Kazakov A, Willemsen J, Bucher D, Bender S, Bartenschlager R, Binder M, Boulant S (2016). Reovirus intermediate subviral particles constitute a strategy to infect intestinal epithelial cells by exploiting TGF-β dependent pro-survival signaling. Cell Microbiol. 18(12):1831-1845

Odendall C, Dixit E, Stavru F, Bierne H, Franz KM, Durbin AF, Boulant S, Gehrke L, Cossart P, Kagan JC (2014). Diverse intracellular pathogens activate type III interferon expression from peroxisomes. Nat Immunol. 8:717–726

Blaising J, Lévy PL, Gondeau C, Varbanov M, Teissier E, Phelip C, Ruggiero F, Polyak SJ, Oberlies NH, Ivanovic T, Boulant S*, Pécheur EI* (2013). Silibinin inhibits hepatitis C virus entry into hepatocytes by hindering clathrin-dependent trafficking. Cell Microbiol. 11:1866-1882 

Boulant S, Stanifer M, Kural C, Cureton DK, Massol R, Whelan S, Nibert ML, Kirchhausen T (2013). Similar uptake but different trafficking and escape routes of reovirus virions and ISVPs imaged in polarized MDCK cells. Mol Biol Cell. 24:1196-1207

Cocucci E, Aguet F, Boulant S, Kirchhausen T (2012). The first 10 seconds in the life of a clathrin coated pit. Cell. 150:495-507

Boulant S*, Ivanovic T*, Ehrlich M, Arnold MM, Kirchhausen T, Nibert ML (2011). Recruitment of cellular clathrin to viral factories by mammalian orthoreovirus protein μNS. Traffic 9:1179-1195

Boulant S, Kural C, Zeeh JC, Ubelmann F, Kirchhausen T (2011). Actin provides additional force to counteract membrane tension during clathrin-mediated endocytosis. Nat Cell Biol. 13:1124-1131

Dixit E, Boulant S, Zhang Y, Lee AS, Odendall C, Shum B, Hacohen N, Chen ZJ, Whelan SP, Fransen M, Nibert ML, Superti-Furga G, Kagan JC (2010). Peroxisomes and mitochondria cooperate to induce antiviral innate immunity. Cell 141:668-681

Boulant S, Douglas M, Moody L, Budkowska A, Targett-Adams P, McLauchlan J (2008). Hepatitis C virus core protein induces lipid droplet redistribution in a microtubule- and dynein-dependent manner. Traffic 8:1268-1282

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