Prof. Dr. Edward Lemke

Synthetic Biophysics of Protein Disorder
Biocentre, Departments Biology and Chemistry
Johannes Gutenberg-University Mainz
Hanns-Dieter-Hüsch-Weg 15
55128 Mainz, Germany

Phone: +49 (0)6131 39-36117

until 2020:

The European Molecular Biology Laboratory
(EMBL) Heidelberg
69117 Heidelberg, Germany


Biophysics, intrinsically disordered proteins, physiochemical properties of biopolymers, single molecule/superresolution/FRET microscopy, intrinsically disordered proteins, protein structure and folding, nucleocytoplasmic shuttling, chemical biology and bioorthogonal labeling reactions


2019 ERC Advanced Grant (LS domain)
2018 Gutenberg Research College Fellow
2017 FEBS anniversary award
2016 Chica and Heinz Schaller award for biomedical research
2015 Young Investigator Award of the International Society for Chemical Biology
2015 ERC consolidator grant SPMFv2.0
2013 MRN Innovation Award
2010-2016 Emmy Noether programme/group leader of the DFG
2006-2008 Feodor-Lynen postdoctoral fellowship, Alexander von Humboldt Foundation
2003-2005 PhD fellowship of the Boehringer Ingelheim Fond
2002 Erwin-Stephan award for excellent and fast studies, TU Berlin
2001 Scholarship of the German National Merit Foundation (Studienstiftung des Deutschen Volkes) until end of university studies
1999-2000 Oversea-scholarship of the Technical University of Berlin
1998 Klaus-Koch-scholarship for excellent studies, Technical University of Berlin
since 2018 Adjunct Director, Institute of Molecular Biology (IMB) and Professor of Synthetic Biophysics, Johannes Gutenberg University (JGU), Mainz
2010-2016 Emmy Noether Group leader
2009-2020 Independent Group leader at the EMBL Heidelberg, Structural and Computational Biology Unit and Cell Biology and Biophysics Unit
2005-2008 Joint Research Associate Department of Chemistry and Department of Molecular Biology at The Scripps Research Institute, La Jolla, CA, USA
2005 PhD (Dr. rer. nat.) Max Planck Insitute for Biophysical Chemistry (and University of Goettingen), Department Membrane Biophysics
2004 Marine Biological Laboratory (Statistics Summerschool)
2002 Diploma in Chemistry, Technical University of Berlin
2001 Master of Science, University of Oklahoma
1999 Vordiploma (BSc) in Chemistry, Technical University of Berlin
2017 Organizer EMBO/EMBL Symposium on Liquid-Liquid Phase separation w018
2017 Organizer EMBO Chemical Biology conference 2018
2014 Organizer EMBO Chemical Biology conference 2014
2011-2016 Module Coordinator EMBL Predoc Course
2011 Organizer EMBO Methods in Chemical Biology Practical course 2011
2010-present Advanced Light Microscopy Facility Steering Committee
2009 Organizer EMBL Light microscopy Workshop

Sakin V, Hanne J, Dunder J, Anders-Osswein M, Laketa V, Nikic I, Krausslich H-G, Lemke EA, Muller B* (2017). A Versatile Tool for Live-Cell Imaging and Super-Resolution Nanoscopy Studies of HIV-1 Env Distribution and Mobility. Cell Chemical Biology 24(5):635-645.e5

Nikić I, Estrada Girona G, Kang JH, Paci G, Mikhaleva S, Koehler C, Shymanska NV, Ventura Santos C, Spitz D, Lemke EA* (2016). Debugging Eukaryotic Genetic Code Expansion for Site-Specific Click-PAINT Super-Resolution Microscopy. Angew Chem Int Ed Engl. 55(52):16172-16176

Koehler C, Sauter PF, Wawryszyn M, Estrada Girona G, Gupta K, Landry JJM, Hsi‐Yang Fritz M, Radic K, Hoffmann J-E, Chen ZA, Zou J, Tan PS, Galik B, Junttila S, Stolt‐Bergner P, Pruneri G, Gyenesei A, Schultz C, Biskup MB, Besir H, Benes V, Rappsilber J, Jechlinger M, Korbel JO, Berger I, Braese S, Lemke EA* (2016). Genetic code expansion for multiprotein complex Engineering. Nature Methods 13(12):997-1000

Milles S, Mercadante D, Aramburu IV, Jensen MR, Banterle B, Koehler C, Tyagi S, Clarke J, Shammas S, Blackledge M*, Gräter F*, Lemke EA* (2015). Plasticity of an ultrafast interaction between nucleoporin and transport receptors. Cell, 10.1016/j.cell.2015.09.047

Nikic I, Plass T, Schraidt O, Szymanski J, Briggs JA, Schultz C, Lemke EA* (2014). Dual-color super-resolution microscopy via genetic code expansion technology and tuned click reactions. Angew Chem Int Ed Engl 53(8), 2245-2249

Milles S, Bui H, Koehler C, Beck M*, Lemke EA* (2012). Facilitated aggregation propensity of FG Nucleoporins under molecular crowding conditions. EMBO Reports 14(2):178-83

Ori A, Banterle N, Iskar M, Andres-Pons A, Escher C, Bui HK, Sparks L, Mezarino VMS, Rinner O, Bork P*, Lemke EA*, Beck M* (2013). Cell-type specific nuclear pore complexes: A case in point for context-dependent stoichiometry of molecular machines. Molecular System Biology 9:648

Plass T, Milles S, Koehler C, Szymanski J, Mueller R, Schultz C*, Lemke EA* (2012). Amino acids for Diels-Alder reactions in living cells. Angew Chem Int Ed Engl 51: 4166-4170

Milles S, Tyagi S, Banterle N, Koehler C, VanDelinder V, Neal A, Lemke EA (2012). Click strategies for single-molecule protein fluorescence. J Am Chem Soc  134: 5187-5195

Plass T, Milles S, Koehler C, Schultz C*, Lemke EA* (2011). Genetically encoded copper-free click chemistry. Angew Chem Int Ed Engl 50: 3878-3881

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